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Depending upon focus, the analyte molecules could undergo affiliation, dissociation and interplay with the solvent to provide a product with different absorption characteristics. For instance, phenol pink undergoes a resonance transformation when shifting from the acidic type to the basic kind . Due to this resonance, the electron distribution of the bonds of molecule changes with the pH of the solvent in which it’s dissolved.

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As long the rise is noticeable you possibly can fit a (non-linear) curve. 2 pairs of footwear, the query was, can we use Lambert-Beer law for suspensions – yes we can. Is a straight ahead theoretical calculation possible, is another query. Istrumental deviations might lead to absorbances which might be smaller than theoretical. Radiation exiting from a monochromator is contaminated with small amounts of scattered or stray radiation, because of scattering and reflections from internal surfaces. When measurements are made within the presence of stray radiation, or with polycromatic radiation , adverse deviations from Beer’s Law happen, specially for greater concentrations.

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