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For very excessive concentrations, the ratio I / I_0 approaches 0, so hardly any mild is transmitted. At very low concentrations, the difference of these values will get so small, that their ratio approaches 1, which suggests the noticed transmittance approaches 1. Usually, if you attain the limits of Beers legislation, you encounter problems along with your instrument sensitiity. Since UV-visible absorption is an digital phenomenon, excessive concentrations would possibly lead to a shift in the absorption wavelength of the analyte.

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At low concentrations, lower than 0.04 the measured has to much error, this results in necessary precision of the absorbance measurement. I Guess, A a part of the absorbed radiation will be emitted again by self quencing , so we’ll get eranius end result on greater concentration.

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